Publicaciones

Hydrologic Landscape Characterization for the Pacific Northwest, USA.

Leibowitz, S., Comeleo, R., Wigington, P., Weber, M., Sproles, E., & Sawicz, K.

We update the Wigington et al. (2013) hydrologic landscape (HL) approach to make it more broadly applicable and apply the revised approach to the Pacific Northwest (PNW; i.e., Oregon, Washington, and Idaho). Specific changes incorporated are the use of assessment units based on National Hydrography Dataset Plus V2 catchments, a modified snowmelt model validated over a broader area, an aquifer permeability index that does not require preexisting aquifer permeability maps, and aquifer and soil permeability classes based on uniform criteria. Comparison of Oregon results for the revised and original approaches found fewer and larger assessment units, loss of summer seasonality, and changes in rankings and proportions of aquifer and soil permeability classes. Differences could be explained by three factors: an increased assessment unit size, a reduced number of permeability classes, and use of smaller cutoff values for the permeability classes. The distributions of the revised HLs in five groups of Oregon rivers were similar to the original HLs but less variable. The improvements reported here should allow the revised HL approach to be applied more often in situations requiring hydrologic classification and allow greater confidence in results. We also apply the map results to the development of hydrologic landscape regions.

Año: 2016

Palabras claves: Hydrologic classification; hydrologic cycle; watersheds; rivers/streams; runoff; geospatial analysis; National Hydrography Dataset, NHD; Pacific Northwest.

Effects of temperature and ocean acidification on shell characteristics of Argopecten purpuratus: implications for scallop aquaculture in an upwelling-influenced area.

Lagos, N., Benítez, S., Duarte, C., Lardies, M., Broitman, B., & Tapia, C. et al.

Coastal upwelling regions already constitute hot spots of ocean acidification as naturally acidified waters are brought to the surface. This effect could be exacerbated by ocean acidification and warming, both caused by rising concentrations of atmospheric CO2. Along the Chilean coast, upwelling supports highly productive fisheries and aquaculture activities. However, during recent years, there has been a documented decline in the national production of the native scallop Argopecten purpuratus. We assessed the combined effects of temperature and pCO2-driven ocean acidification on the growth rates and shell characteristics of this species farmed under the natural influence of upwelling waters occurring in northern Chile (30°S, Tongoy Bay). The experimental scenario representing current conditions (14°C, pH ~8.0) were typical of natural values recorded in Tongoy Bay, whilst conditions representing the low pH scenario were typical of an adjacent upwelling area (pH ~7.6). Shell thickness, weight, and biomass were reduced under low pH (pH ~7.7) and increased temperature (18°C) conditions. At ambient temperature (14°C) and low pH, scallops showed increased shell dissolution and low growth rates. However, elevated temperatures ameliorated the impacts of low pH, as evidenced by growth rates in both pH treatments at the higher temperature treatment that were not significantly different from the control treatment. The impact of low pH at current temperature on scallop growth suggests that the upwelling could increase the time required for scallops to reach marketable size. Mortality of farmed scallops is discussed in relation to our observations of multiple environmental stressors in this upwelling-influenced area.

Año: 2016

Palabras claves: Calcification, Shell growth, Scallop farming, Upwelling, Chile.

South Pacific Integrated Ecosystem Studies meeting: toward conservation and sustainable use of marine resources in the South Pacific.

Parada, C., Frusher, S., Bustamante, R., Di Lorenzo, E., Bernal, P., & Cryer, M.Dunn, A., Garreaud, R., Gutierrez, M., Jennings, S., Montecinos, A., Neira, S., Quiñones, R.A., Takahashi, K., Tascheri, R., Yannicelli, B.

The South Pacfic region represents the world’s largest oceanic water mass and plays a signficant role in the earth’s climate systems. This region also contains the
largest group of island nations, most of whom are dependent on marine resources for their livelihoods. Several of the largest coastal and oceanic fisheries also
occur in this region (FAO, 2014). In addition, for the countries associated with the southern Pacfic Ocean region, the sea provides signficant social , cultural and
economic benfits, with many countries being heavily reliant on both coastal and oceanic marine resources (Bell et al., 2013). Increasing coastal populations and
climate change are expected to augment human demands on already fully exploited or over-exploi ted marine resources, threatening both food security and
sustainable livelihoods (Bell et al., 2011). Therefore, it is imperative that the science that describes and predicts linked biophysical and human systems is understood and developed to meet these needs. Climate change will have many impacts on marine ecosystems, with implications for end users including individuals, local communities, industries and governments. Improved scientfic support for policy and management decision-making in the face of these potential impacts is essential.

Año: 2016

Palabras claves:

Molecular characterization of an inhibitor of NF-κB in the scallop Argopecten purpuratus: First insights into its role on antimicrobial peptide regulation in a mollusk.

Oyanedel, D., Gonzalez, R., Flores-Herrera, P., Brokordt, K., Rosa, R., Mercado, L., & Schmitt, P.

Inhibitors of nuclear factor kappa B (IκBs) are major control components of the Rel/NF-κB signaling pathway, a key regulator in the modulation of the expression of immune-related genes in vertebrates and invertebrates. The activation of the Rel/NF-κB signaling pathway depends largely in the degradation of IκB proteins and thus, IκBs are a main target for the identification of genes whose expression is controlled by Rel/NF-κB pathway. In order to identify such regulation in bivalve mollusks, the cDNA sequence encoding an IκB protein was characterized in the scallop Argopecten purpuratus, ApIκB. The cDNA sequence of ApIκB is comprised of 1480 nucleotides with a 1086 bp open reading frame encoding for 362 amino acids. Bioinformatics analysis showed that ApIκB displays the conserved features of IκB proteins. The deduced amino acid sequence consists of a 39.7 kDa protein, which has an N-terminal degradation motif, six ankyrin repeats and a C-terminal phosphorylation site motif. Phylogenetic analysis revealed a high degree of identity between ApIκB and other IκBs from mollusks, but also to arthropod cactus proteins and vertebrate IκBs. Tissue expression analysis indicated that ApIκB is expressed in all examined tissues and it is upregulated in circulating hemocytes from scallops challenged with the pathogenic Gram-negative bacterium Vibrio splendidus. After inhibiting ApIκB gene expression using the RNA interference technology, the gene expression of the antimicrobial peptide big defensin was upregulated in hemocytes from non-challenged scallops. Results suggest that ApIκB may control the expression of antimicrobial effectors such as big defensin via a putative Rel/NF-κB signaling pathway. This first evidence will help to deepen the knowledge of the Rel/NF-κB conserved pathway in scallops.

Año: 2016

Palabras claves: Signaling pathway; NF-κB pathway; Inhibition of nuclear factor kappa B; Antimicrobial peptide; Big defensin; Immunoregulation; Scallop; RNA interference.

Variability of 14C reservoir age and air–sea flux of CO2 in the Peru–Chile upwelling region during the past 12,000years.

Carré, M., Jackson, D., Maldonado, A., Chase, B., & Sachs, J.

The variability of radiocarbon marine reservoir age through time and space limits the accuracy of chronologies in marine paleo-environmental archives. We report here new radiocarbon reservoir ages (ΔR) from the central coast of Chile (~ 32°S) for the Holocene period and compare these values to existing reservoir age reconstructions from southern Peru and northern Chile. Late Holocene ΔR values show little variability from central Chile to Peru. Prior to 6000 cal yr BP, however, ΔR values were markedly increased in southern Peru and northern Chile, while similar or slightly lower-than-modern ΔR values were observed in central Chile. This extended dataset suggests that the early Holocene was characterized by a substantial increase in the latitudinal gradient of marine reservoir age between central and northern Chile. This change in the marine reservoir ages indicates that the early Holocene air–sea flux of CO2 could have been up to five times more intense than in the late Holocene in the Peruvian upwelling, while slightly reduced in central Chile. Our results show that oceanic circulation changes in the Humboldt system during the Holocene have substantially modified the air–sea carbon flux in this region.

Año: 2016

Palabras claves: Humboldt system; Reservoir age; Shell middens; Deglaciation; Radiocarbon; CO2.

Climate variability and human impact in South America during the last 2000 years: synthesis and perspectives from pollen records.

Flantua, S., Hooghiemstra, H., Vuille, M., Behling, H., Carson, J., & Gosling, W., Hoyos, I., Ledru, M.P., Montoya, E., Mayle, F., Maldonado, A. et al.

An improved understanding of present-day climate variability and change relies on high-quality data sets from the past 2 millennia. Global efforts to model regional climate modes are in the process of being validated against, and integrated with, records of past vegetation change. For South America, however, the full potential of vegetation records for evaluating and improving climate models has hitherto not been sufficiently acknowledged due to an absence of information on the spatial and temporal coverage of study sites. This paper therefore serves as a guide to high-quality pollen records that capture environmental variability during the last 2 millennia. We identify 60 vegetation (pollen) records from across South America which satisfy geochronological requirements set out for climate modelling, and we discuss their sensitivity to the spatial signature of climate modes throughout the continent. Diverse patterns of vegetation response to climate change are observed, with more similar patterns of change in the lowlands and varying intensity and direction of responses in the highlands. Pollen records display local-scale responses to climate modes; thus, it is necessary to understand how vegetation–climate interactions might diverge under variable settings. We provide a qualitative translation from pollen metrics to climate variables. Additionally, pollen is an excellent indicator of human impact through time. We discuss evidence for human land use in pollen records and provide an overview considered useful for archaeological hypothesis testing and important in distinguishing natural from anthropogenically driven vegetation change. We stress the need for the palynological community to be more familiar with climate variability patterns to correctly attribute the potential causes of observed vegetation dynamics. This manuscript forms part of the wider LOng-Term multi-proxy climate REconstructions and Dynamics in South America – 2k initiative that provides the ideal framework for the integration of the various palaeoclimatic subdisciplines and palaeo-science, thereby jump-starting and fostering multidisciplinary research into environmental change on centennial and millennial timescales.

Año: 2016

Palabras claves:

Terral de Vicuña, a Foehnlike Wind in Semiarid Northern Chile: Meteorological Aspects and Implications for the Fulfillment of Chill Requirements in Deciduous Fruit Trees.

Montes, C., Rutllant, J., Aguirre, A., Bascuñán-Godoy, L., & Juliá, C.

The terral de Vicuña is a warm and dry wind that flows down the Elqui Valley in north-central Chile typically at dawn and early morning. Given that most terral episodes occur in austral winter when chill accumulation by deciduous fruit trees proceeds, negative effects on agriculture may be expected. During 11 (2004–14) winters a meteorological characterization of terral winds and the assessment of their impact on chill accumulation, by the modified Utah Model and the Dynamic Model, were performed. Within this period, 67 terral days (TD) were identified as those in which nighttime to early morning wind direction and speed, air temperature, and relative humidity reached defined thresholds on an hourly basis (terral hours). Most frequent TD featured 6–9 consecutive terral hours; duration is considered here as a proxy for their intensity. Synoptic-scale meteorological analysis shows that 65% of moderate and strong terral events develop as a cold, migratory anticyclone drifts poleward of the study area, coinciding with the onset of a midtropospheric ridge over central Chile, bringing southwest winds on top of the Andes (~500-hPa level). The remaining 35% are either associated with 500-hPa easterlies (foehn like), with prefrontal conditions ahead of a trough driving northwest 500-hPa winds, or with transitional 500-hPa westerlies. Assessments of chill accumulation during TD show that, although present average and cold winter conditions do not represent a major TD hazard to local agriculture, lower chill accumulation associated with anomalously high nocturnal temperatures could be significantly more important during present and future warmer winters.

Año: 2016

Palabras claves: South America; Downslope winds; Regional effects; Ecological models; Agriculture.

Developing a Snowmelt Forecast Model in the Absence of Field Data.

Sproles, E., Kerr, T., Orrego Nelson, C., & Lopez Aspe, D.

In data poor regions predicting water availability is a considerable challenge for water resource managers. In snow-dominated watersheds with minimal in situ measurements, satellite imagery can supplement sparse data networks to predict future water availability. This technical note presents the first phase of an operational forecast model in the data poor Elqui River watershed located in northern Central Chile (30°S). The approach applies remotely-sensed snow cover products from the Moderate Resolution Imaging Spectrometer (MODIS) instrument as the first order hydrologic input for a modified Snowmelt Runoff Model. In the semi-arid Elqui River, snow and glacier melt are the dominant hydrologic inputs but precipitation is limited to up to six winter events annually. Unfortunately winter access to the Andean Cordillera where snow accumulates is incredibly challenging, and thus measurements of snowpack are extremely sparse. While a high elevation snow monitoring network is under development, management decisions regarding water resources cannot wait as the region is in its eighth consecutive year of drought. Our model applies a Monte Carlo approach on monthly data to determine relationships between lagged changes in snow covered area and previous streamflow to predict subsequent streamflow. Despite the limited data inputs the model performs well with a Nash-Sutcliffe Efficiency and R2 of 0.830 and 0.833 respectively. This model is not watershed specific and is applicable in other regions where snow dominates hydrologic inputs, but measurements are minimal.

Año: 2016

Palabras claves: Snowmelt runoff, Hydrological model, Remote sensing, Hydrological prediction.

Molecular characterization of two ferritins of the scallop Argopecten purpuratus and gene expressions in association with early development, immune response and growth rate.

Coba de la Peña, T., Cárcamo, C., Díaz, M., Brokordt, K., & Winkler, F.

Ferritin is involved in several iron homoeostasis processes in molluscs. We characterized two ferritin homologues and their expression patterns in association with early development, growth rate and immune response in the scallop Argopecten purpuratus, a species of economic importance for Chile and Peru. Two ferritin subunits (Apfer1 and Apfer2) were cloned. Apfer1 cDNA is a 792 bp clone containing a 516 bp open reading frame (ORF) that corresponds to a novel ferritin subunit in A. purpuratus. Apfer2 cDNA is a 681 bp clone containing a 522 bp ORF that corresponds to a previously sequenced EST. A putative iron responsive element (IRE) was identified in the 5′-untranslated region of both genes. The deduced protein sequences of both cDNAs possessed the motifs and domains characteristic of functional ferritin subunits. Both genes showed differential expression patterns at tissue-specific and early development stage levels. Apfer1 expression level increased 40-fold along larval developmental stages, decreasing markedly after larval settlement. Apfer1 expression in mantle tissue was 2.8-fold higher in fast-growing than in slow-growing scallops. Apfer1 increased 8-fold in haemocytes 24 h post-challenge with the bacterium Vibrio splendidus. Apfer2 expression did not differ between fast- and slow-growing scallops or in response to bacterial challenge. These results suggest that Apfer1 and Apfer2 may be involved in iron storage, larval development and shell formation. Apfer1 expression may additionally be involved in immune response against bacterial infections and also in growth; and thus would be a potential marker for immune capacity and for fast growth in A. purpuratus.

Año: 2016

Palabras claves: Ferritin; Argopecten purpuratus; Iron homoeostasis; Growth; Development; Immunity, Apfer1, Apfer2.