Publicaciones
Molecular phylogeny of hinge-beak shrimps (Decapoda: Caridea: Rhynchocinetes and Cinetorhynchus ) and allies: a formal test of familiar and generic monophyly using a multilocus phylogeny.
Baeza, J., Bauer, R., Okuno, J., & Thiel, M.
The Rhynchocinetidae (‘hinge-beak’ shrimps) is a family of marine caridean decapods with considerable variation in sexual dimorphism, male weaponry, mating tactics, and sexual systems. Thus, this group is an excellent model with which to analyse the evolution of these important characteristics, which are of interest not only in shrimps specifically but also in animal taxa in general. Yet, there exists no phylogenetic hypothesis, either molecular or morphological, for this taxon against which to test either the evolution of behavioural traits within the Rhynchocinetidae or its genealogical relationships with other caridean taxa. In this study, we tested (1) hypotheses on the phylogenetic relationships of rhynchocinetid shrimps, and (2) the efficacy of different (one-, two-, and three-phase) methods to generate a reliable phylogeny. Total genomic DNA was extracted from tissue samples taken from 17 species of Rhynchocinetidae and five other species currently or previously assigned to the same superfamily (Nematocarcinoidea); six species from other superfamilies were used as outgroups. Sequences from two nuclear genes (H3 and Enolase) and one mitochondrial gene (12S) were used to construct phylogenies. One-phase phylogenetic analyses (SATé-II) and classical two- and three-phase phylogenetic analyses were employed, using both maximum likelihood and Bayesian inference methods. Both a two-gene data set (H3 and Enolase) and a three-gene data set (H3, Enolase, 12S) were utilized to explore the relationships amongst the targeted species. These analyses showed that the superfamily Nematocarcinoidea, as currently accepted, is polyphyletic. Furthermore, the two major clades recognized by the SATé-II analysis are clearly concordant with the genera Rhynchocinetes and Cinetorhynchus, which are currently recognized in the morphological-based classification (implicit phylogeny) as composing the family Rhynchocinetidae. The SATé-II method is considered superior to the other phylogenetic analyses employed, which failed to recognize these two major clades. Studies using more genes and a more complete species data set are needed to test yet unresolved inter- and intrafamilial systematic and evolutionary questions about this remarkable clade of caridean shrimps. © 2014 The Linnean Society of London
Año: 2014
Palabras claves: Bayesian hypothesis testing; Crustacea; SATé-II.
Seed predation by rodents results in directed dispersal of viable seed fragments of an endangered desert shrub.
Loayza, A., Carvajal, D., García-Guzmán, P., Gutierrez, J., & Squeo, F.
Seed predation and seed dispersal are important ecological processes with antagonistic effects on plant recruitment. In the southern edge of the Atacama Desert in Chile, Myrcianthes coquimbensis is an endangered, large-seeded, vertebrate-dispersed shrub that in the present-day has no known dispersers. Native rodents hoard and eat the seeds of M. coquimbensis but leave viable seed fragments at the hoarding sites; soil interspaces within rock outcrops where seedlings recruit. Here we examined whether rodents act as effective dispersers of M. coquimbensis by discarding viable seed fragments in sites suitable for recruitment. We simulated different levels of endosperm loss to determine if seedlings could develop from seed fragments. We assessed how frequently rodents discarded fragments, and the probability that these fragments produced seedlings. Finally, we compared emergence and seedling survival at the hoarding sites and in two other habitats where seeds arrive to evaluate the suitability of the hoarding sites. Seeds of M. coquimbensis developed seedlings even after 87% of their storage tissue was removed. Rodents left seed fragments in more than 50% of the trials; almost 60% of the discarded fragments produced seedlings. Seedlings did not emerge from open ground habitats, and emergence was higher under M. coquimbensis shrubs than in rock habitats. Survival of two-year-old seedlings was higher in rock habitats than under conspecific adult shrubs. Our results suggest that rodents may play a dual role in the recruitment dynamics of M. coquimbensis, acting simultaneously as seed predators and effective dispersers. Therefore, though seed predators impose costs, their net effect on plant fitness in this system—where dispersers of large-seeded species have been lost—is likely positive.
Año: 2014
Palabras claves: Anachronism; Atacama; captive feeding trials; hoarding; Myrcianthes coquimbensis; rodent; seedling establishment.
Functional traits determine plant co-occurrence more than environment or evolutionary relatedness in global drylands.
Soliveres, S., Maestre, F., Bowker, M., Torices, R., Quero, J., & García-Gómez, M., Cabrera, O., Ceag, A.P., Coaguila, D., Eldridge D.J., Espinosa, C.I., Hemmings, F., Monerris, J.J., Tighe, M., Delgado-Baquerizo, M., Escolar, C., García-Palacios, P., Gozalo, B., Ochoa, V., Blones, J., Derak, M., Ghiloufi, W., Gutiérrez, J.R., Hernández, R.M., Noumi, Z.
Plant–plant interactions are driven by environmental conditions, evolutionary relationships (ER) and the functional traits of the plants involved. However, studies addressing the relative importance of these drivers are rare, but crucial to improve our predictions of the effects of plant–plant interactions on plant communities and of how they respond to differing environmental conditions. To analyze the relative importance of – and interrelationships among – these factors as drivers of plant–plant interactions, we analyzed perennial plant co-occurrence at 106 dryland plant communities established across rainfall gradients in nine countries. We used structural equation modelling to disentangle the relationships between environmental conditions (aridity and soil fertility), functional traits extracted from the literature, and ER, and to assess their relative importance as drivers of the 929 pairwise plant–plant co-occurrence levels measured. Functional traits, specifically facilitated plants’ height and nurse growth form, were of primary importance, and modulated the effect of the environment and ER on plant–plant interactions. Environmental conditions and ER were important mainly for those interactions involving woody and graminoid nurses, respectively. The relative importance of different plant–plant interaction drivers (ER, functional traits, and the environment) varied depending on the region considered, illustrating the difficulty of predicting the outcome of plant–plant interactions at broader spatial scales. In our global-scale study on drylands, plant–plant interactions were more strongly related to functional traits of the species involved than to the environmental variables considered. Thus, moving to a trait-based facilitation/competition approach help to predict that: (1) positive plant–plant interactions are more likely to occur for taller facilitated species in drylands, and (2) plant–plant interactions within woody-dominated ecosystems might be more sensitive to changing environmental conditions than those within grasslands. By providing insights on which species are likely to better perform beneath a given neighbour, our results will also help to succeed in restoration practices involving the use of nurse plants.
Año: 2014
Palabras claves: Aridity; Competition; Facilitation; Phylogenetic distance; Semi-arid; Soil fertility.
Climate and soil attributes determine plant species turnover in global drylands.
Ulrich, W., Soliveres, S., Maestre, F., Gotelli, N., Quero, J., & Delgado-Baquerizo, M. Bowker, M.A., Eldridge, D.J., Ochoa, V., Gozalo, B., Valencia, E., Berdugo, M., Escolar, C., García-Gómez, M., Escudero, A., Prina, A., Alfonso, G., Arredondo, T., Bran, D., Cabrera, O., Cea, A.P., Chaieb, M., Contreras, J., Derak, M., Espinosa, C.I., Florentino, A., Gaitán, J., García Muro, V., Ghiloufi, W., Gómez-González, S., Gutiérrez, J.R., et al.
Geographical, climatic and soil factors are major drivers of plant beta diversity, but their importance for dryland plant communities is poorly known. The aim of this study was to: (1) characterize patterns of beta diversity in global drylands; (2) detect common environmental drivers of beta diversity; and (3) test for thresholds in environmental conditions driving potential shifts in plant species composition.
Año: 2014
Palabras claves: Aridity; beta diversity; climatic variability; global environmental change; habitat filtering; latitudinal gradient; plant community assembly; regression analysis; soil fertility; spatial soil heterogeneity.
Environmental tolerance of the two invasive species Ciona intestinalis and Codium fragile: their invasion potential along a temperate coast.
Jofré Madariaga, D., Rivadeneira, M., Tala, F., & Thiel, M.
Ciona intestinalis and Codium fragile are among the most successful invasive species in marine systems worldwide, and they are currently in the process of expanding their distributional ranges along the Chilean coast. Herein we evaluated whether their tolerance to a wide range of environmental conditions contributes to the invasion potential of these two species. To examine the environmental tolerance and performance (e.g., growth) of these non-indigenous species, unifactorial experiments (8–10 days) were conducted with different environmental factors (solar radiation, salinity and temperature). Based on the results, the potential risk of invasion along the Chilean coast was evaluated for both species using a mechanistic niche modeling approach. Both species can tolerate extensive ranges of the abiotic factors salinity and temperature, with C. intestinalis being more tolerant to cold-temperate waters. Also, C. intestinalis was more susceptible to high light intensities than C. fragile. These results confirm those of other experiments, and the outcome of the niche modeling shows that both species can potentially invade most regions of the Chilean coast with the exception of the Magellan region. The results suggest that physiological capacity to tolerate and perform in a wide range of physical conditions is a pre-requisite for successful invasions by littoral biota, but predation and possibly competitive exclusion can slow down the invasion success of C. intestinalis, which in contrast to C. fragile, is consumed by many benthic predators. Sexual and asexual reproduction as well as buoyancy of its thalli further contribute to the dispersal and colonization success of C. fragile. Based on these considerations, it is concluded that the invasion risk of C. fragile along the Chilean coast is substantially higher than that of C. intestinalis.
Año: 2014
Palabras claves: Invasive species, Environmental tolerance, Chilean coastInvasion risk, Niche modeling.
Effect of grazing on distribution and recovery of harvested stands of Lessonia berteroana kelp in northern Chile.
Oróstica, M., Aguilera, M., Donoso, G., Vásquez, J., & Broitman, B.
Understanding the ecological factors regulating exploited natural communities is important in establishing conservation and management strategies. Since the 1960s, artisanal fishermen have harvested up to 300000 dry tons yr-1 of wild populations of Lessonia spp. kelps. Adult kelps form a key habitat on the rocky shores of Chile, and benthic grazers regulate kelp populations by grazing or bulldozing microscopic and juvenile stages. To establish the role of the grazer assemblage in the recovery of kelp stands following harvesting, we conducted a manipulative experiment simulating artisanal kelp extraction and then manipulating the presence of grazers. We followed community succession and spatial distribution of L. berteroana recruits in manipulated and control rocky shore platforms for ca. 12 mo. Inter-individual distances of recruits and spatial autocorrelation analyses showed that the presence of grazers determined a patchy distributional pattern of L. berteroana. The aggregated spatial pattern of kelp recruits in the presence of grazers was followed by numerous coalescence events between small holdfasts, but no coalescence events were observed between recruits in the grazer removal areas. Our results suggest that grazing and recruit coalescence play an important role in the recovery of kelp stands following artisanal harvesting. Incorporating these processes into conservation and management strategies may bolster current strategies, which are based solely on the spatial structure of kelp stands.
Año: 2014
Palabras claves: Algae-herbivore, Harvesting, Grazers, Kelp communities, Spatial structure.
Spatial variability in community composition on a granite breakwater versus natural rocky shores: Lack of microhabitats suppresses intertidal biodiversity.
Aguilera, M., Broitman, B., & Thiel, M.
Strong differences have been observed between the assemblages on artificial reefs and on natural hard-bottom habitats worldwide, but little is known about the mechanisms that cause contrasting biodiversity patterns. We examined the influence of spatial attributes in relation to both biogenic and topographic microhabitats, in the distribution and composition of intertidal species on both artificial and natural reefs. We found higher small-scale spatial heterogeneity on the natural reef compared with the study breakwater. Species richness and diversity were associated with a higher availability of crevices, rock pools and mussels in natural habitats. Spatial distribution of certain grazers corresponded well with the spatial structure of microhabitats. In contrast, the lack of microhabitats on the breakwater resulted in the absence of several grazers reflected in lower species richness. Biogenic and topographic microhabitats can have interactive effects providing niche opportunities for multiple species, explaining differences in species diversity between artificial versus natural reefs.
Año: 2014
Palabras claves: Artificial reefs; Biodiversity; Breakwater; Grazers; Spatial heterogeneity; Microhabitats.
Hongos endófitos antárticos como herramienta para la reintroducción de especies nativas en zonas áridas.
Fardella, C., Oses, R., Torres-Díaz, C., & Molina-Montenegro, M.
Ecological restoration is the deliberate action that speeds recovery from a degraded system by direct or indirect human intervention. Xerophytic formations are among the most degraded ecosystems where low water availability makes reintroduction plans unsuccessful and extremely expensive. Roots inoculation with endophytic fungi has been reported as a successful strategy to maintain or improve the ecophysiological performance and survival in different species of shrubs and trees. In harsh environments, endophytes have been shown to provide benefits in terms of survival and growth for their associated vegetation. In this study, we showed that inoculation with endophytes isolated from plants growing in Antarctica improve survival and water use efficiency for irrigation on native species of xerophytic formations: Flourensia thurifera, Senna cumingii and Puya berteroniana. Overall, the presence of endophytes improved survival of all native species. Furthermore, the presence of endophytes improved water use efficiency for irrigation, reaching higher percentages of survival in plants with a reduction in the added water. The application of Antarctic endophytes could be considered a successful strategy for future projects and reintroduction programs in arid and semi-arid zones of Chile.
Año: 2014
Palabras claves: Endophytes, water use efficiency, xerophytic formations, restoration.
Economic valuation of kelp forests in northern Chile: values of goods and services of the ecosystem.
Vásquez, J., Zuñiga, S., Tala, F., Piaget, N., Rodríguez, D., & Vega, J.
Kelp beds, besides being one of the most important benthic resources in northern Chile, provide a variety of environmental goods and services. In order to evaluate economically the wild kelp populations in northern Chile (26° to 32° S) more than simply their commercial value as a source of raw materials for alginate extraction, we used several economic indicators to holistically assess the value of a group of brown seaweeds of economic importance, Lessonia spp. and Macrocystis pyrifera: (1) market value of biomass as a source of raw material for extraction of alginic acid, (2) market value of associated species of economic importance, (3) value as a source of scientific information, (4) value as a climate buffer (CO2 capture and release of O2), (5) value of associated biodiversity (non-commercial species), (6) value as cultural heritage and (7) value as a reservoir of biodiversity. Existence values of kelp beds which estimate the willingness of citizens to pay and work without payment to preserve the ecosystem were calculated using the contingent valuation technique. The results indicate that kelp beds in northern Chile have a total value of US $540 million. Of this total, kelp fishery accounts for 75 % and associated-species fisheries account for 15 %. In this context, the economic value of Chilean kelp beds is mainly associated with the industry of alginate extraction. By contrast, existence value as a source of scientific information or environmental buffer for CO2 capture or O2 production represents only 9 % of the total value, representing a very low relative importance to society. The economic valuation of coastal resources and marine ecosystems is a complementary tool for decision making and implementation of public policies related to the conservation and sustainable exploitation of renewable resources and their ecosystems.
Año: 2014
Palabras claves: Kelp fishery, Phaeophyta, Administrative policies, Ecosystem goods and services, Conservation, Economic value.
Morphological, ultrastructural, and genetic characterization of coalescence in the intertidal and shallow subtidal kelps Lessonia spicata and L. berteroana (Laminariales, Heterokontophyta).
González, A., Borras-Chavez, R., Beltrán, J., Flores, V., Vásquez, J., & Santelices, B.
Coalescing macroalgae may fuse with conspecifics, forming genetically heterogeneous entities known as chimera. This process has been shown in taxa from roughly half the red algal orders and in the Codium species, a green alga. Field observations indicate that common and dominant kelps along central Chile exhibit a fused holdfast. We evaluated whether such fusions are true coalescence processes in Lessonia spicata and Lessonia berteroana. To this end, we characterized the ultrastructural event involved in holdfast fusion in the laboratory. Additionally, coalescence in natural populations was quantified by measuring the frequency of individuals with genetically heterogenic stipes within the same holdfast. Results indicate that coalescence appears as a frequent process in laboratory, mostly restricted to intraspecific fusions. During fusion, the meristodermatic cells located in the contact area modify their morphology and reduce the number of plastids, mitochondria, and cell inclusions. The cell wall becomes much thinner and develops plasmodesmata, enhancing communication with equivalent cells of the other coalescencing individual. Stipe genotyping indicates that there is a widespread occurrence of chimerism in both species and genetic heterogeneity is increasing directly with the increasing number of stipes. The combination of results suggests that kelp frequently coalesce in the field, and the histological response observed approaches that of red algae. Since kelps are part of the dominant vegetation in low intertidal and shallow subtidal beds, the adaptive values of coalescence in these species should be evaluated. It is concluded that coalescence and chimerism are evolutionary convergent processes, occurring in all three major groups of seaweeds.
Año: 2014
Palabras claves: Coalescence, Chimerism, Kelps, Lessonia, Microsatellites, Heterokontophyta.