CEAZA
Molecular characterization of two ferritins of the scallop Argopecten purpuratus and gene expressions in association with early development, immune response and growth rate.
Coba de la Peña, T., Cárcamo, C., Díaz, M., Brokordt, K., & Winkler, F.
Ferritin is involved in several iron homoeostasis processes in molluscs. We characterized two ferritin homologues and their expression patterns in association with early development, growth rate and immune response in the scallop Argopecten purpuratus, a species of economic importance for Chile and Peru. Two ferritin subunits (Apfer1 and Apfer2) were cloned. Apfer1 cDNA is a 792 bp clone containing a 516 bp open reading frame (ORF) that corresponds to a novel ferritin subunit in A. purpuratus. Apfer2 cDNA is a 681 bp clone containing a 522 bp ORF that corresponds to a previously sequenced EST. A putative iron responsive element (IRE) was identified in the 5′-untranslated region of both genes. The deduced protein sequences of both cDNAs possessed the motifs and domains characteristic of functional ferritin subunits. Both genes showed differential expression patterns at tissue-specific and early development stage levels. Apfer1 expression level increased 40-fold along larval developmental stages, decreasing markedly after larval settlement. Apfer1 expression in mantle tissue was 2.8-fold higher in fast-growing than in slow-growing scallops. Apfer1 increased 8-fold in haemocytes 24 h post-challenge with the bacterium Vibrio splendidus. Apfer2 expression did not differ between fast- and slow-growing scallops or in response to bacterial challenge. These results suggest that Apfer1 and Apfer2 may be involved in iron storage, larval development and shell formation. Apfer1 expression may additionally be involved in immune response against bacterial infections and also in growth; and thus would be a potential marker for immune capacity and for fast growth in A. purpuratus.
Año: 2016
Palabras claves: Ferritin; Argopecten purpuratus; Iron homoeostasis; Growth; Development; Immunity, Apfer1, Apfer2.
Referencia APA: Coba de la Peña, T., Cárcamo, C., Díaz, M., Brokordt, K., & Winkler, F. (2016). Molecular characterization of two ferritins of the scallop Argopecten purpuratus and gene expressions in association with early development, immune response and growth rate. Comparative Biochemistry And Physiology Part B: Biochemistry And Molecular Biology, 198, 46-56.
3-D surface properties of glacier penitentes over an ablation season, measured using a Microsoft Xbox Kinect.
Nicholson L. I., Pętlicki M., Partan B., and MacDonell S.
In this study, the first small-scale digital surface models (DSMs) of natural penitentes on a glacier surface were produced using a Microsoft Xbox Kinect sensor on Tapado Glacier, Chile (30°08′ S, 69°55′ W). The surfaces produced by the complete processing chain were within the error of standard terrestrial laser scanning techniques, but insufficient overlap between scanned sections that were mosaicked to cover the sampled areas can result in three-dimensional (3-D) positional errors of up to 0.3 m. Between November 2013 and January 2014 penitentes become fewer, wider and deeper, and the distribution of surface slope angles becomes more skewed to steep faces. Although these morphological changes cannot be captured by manual point measurements, mean surface lowering of the scanned areas was comparable to that derived from manual measurements of penitente surface height at a minimum density of 5 m−1 over a 5 m transverse profile. Roughness was computed on the 3-D surfaces by applying two previously published geometrical formulae: one for a 3-D surface and one for single profiles sampled from the surface. Morphometric analysis shows that skimming flow is persistent over penitentes, providing conditions conducive for the development of a distinct microclimate within the penitente troughs. For each method a range of ways of defining the representative roughness element height was used, and the calculations were done both with and without application of a zero displacement height offset to account for the likelihood of skimming air flow over the closely spaced penitentes. The computed roughness values are on the order of 0.01–0.10 m during the early part of the ablation season, increasing to 0.10–0.50 m after the end of December, in line with the roughest values previously published for glacier ice. Both the 3-D surface and profile methods of computing roughness are strongly dependent on wind direction. However, the two methods contradict each other in that the maximum roughness computed for the 3-D surface coincides with airflow across the penitente lineation, while maximum roughness computed for sampled profiles coincides with airflow along the penitente lineation. These findings highlight the importance of determining directional roughness and wind direction for strongly aligned surface features and also suggest more work is required to determine appropriate geometrical roughness formulae for linearized features.
Año: 2016
Palabras claves:
Referencia APA: Nicholson L. I., Pętlicki M., Partan B., and MacDonell S. (2016). 3-D surface properties of glacier penitentes over an ablation season, measured using a Microsoft Xbox Kinect. The Cryosphere, 10(5), 1897.
Late Holocene environmental changes as recorded in the sediments of high Andean Laguna Chepical, Central Chile (32° S; 3050ma. sl).
Martel-Cea, A., Maldonado, A., Grosjean, M., Alvial, I., de Jong, R., Fritz, S. C., & von Gunten, L.
We present a reconstruction of environmental changes from sediments of high-altitude Laguna Chepical in the subtropical Andes of Central Chile (32°16′S; 70°30′W, 3050 m a.s.l.) for the past 3100 years. Based on subfossil pollen, microscopic charcoal and diatoms, we inferred changes in moisture (related to precipitation) and ice-cover/ice-free season (related to summer temperature) at decadal to millennial scales. Sustained wetter and colder summer temperatures than today prevailed between 1100 BC and ca. AD 1. Afterward, decreasing pollen accumulation rates and increased fire activity suggest drier conditions and possibly enhanced seasonality and/or inter-annual climate variability. Frequent changes between cold and warm summers were observed, particularly for the last 1000 years. About AD 1250 (during the Medieval Climate Anomaly), wet years and early break up of ice-cover occurred in central Chile, which is today typical for El Niño-like mean conditions. Conversely, and with the exception of a few wet pulses, a generally dry period with extended ice-cover (cool summers) was observed between AD 1400 and AD 1850 (Little Ice Age). This can be interpreted as a trend toward more La Niña-like mean conditions. Recent climate change and human disturbances during the last 100 years have prompted changes in diatom and plant communities that are unprecedented in the late Holocene. First, planktonic diatoms increased as a result of hydraulic interventions in the lake during the late 19th century, and secondly, the Andean vegetation shifted upward as result of recent warming, and the frequency of arboreal taxa was significantly reduced. At the same time peaks of fire activity were observed.
Año: 2016
Palabras claves: Late Holocene; ENSO; Pollen; Diatom; Andes; Climate change.
Referencia APA: Martel-Cea, A., Maldonado, A., Grosjean, M., Alvial, I., de Jong, R., Fritz, S. C., & von Gunten, L. (2016). Late Holocene environmental changes as recorded in the sediments of high Andean Laguna Chepical, Central Chile (32° S; 3050ma. sl). Palaeogeography, Palaeoclimatology, Palaeoecology, 461, 44-54.
Dairy slurry application to grasslands and groundwater quality in a volcanic soil.
Huertas, J., Cuevas, J. G., Paulino, L., Salazar, F., Arumí, J. L., & Dörner, J.
Research in volcanic-ash soils has shown that they largely capture the dairy slurry following application to land; however, their hydrological properties would favor nutrient leaching. Our objective was to evaluate the contribution of biogeochemical and hydrological controls on the pollution of groundwater by cattle slurry applied to a permanent grassland growing on a volcanic soil. We sampled groundwater chemistry since 10 months before the fertilization (three samplings), and 16 months after, with samplings 1-2 months after the fertigation. Following fertilization, ammonium, exchangeable potassium, and magnesium soil concentrations increased in the fertilized plots compared to the control plots. In contrast, no effect of slurry on groundwater quality was detected, with the exception of dissolved organic nitrogen, a main component of dairy slurry that increased in the groundwater below the fertilized plots. Despite the fact that biogeochemical controls predominate, hydrological aspects would be important when rainfall is high, evapotranspiration is low, groundwater table level is high, and water movement in the saturated zone increases. We concluded that the application of slurry to pastures under rates comparable to a high fertilization in the short term, does not generally impact the groundwater quality in volcanic ash-derived soils.
Año: 2016
Palabras claves: Andisol; groundwater pollution; hydraulic properties; saturated zone; dissolved organic nitrogen.
Referencia APA: Huertas, J., Cuevas, J. G., Paulino, L., Salazar, F., Arumí, J. L., & Dörner, J. (2016). Dairy slurry application to grasslands and groundwater quality in a volcanic soil. Journal of soil science and plant nutrition, 16(3), 745-762.
A 700-year record of climate and environmental change from a high Andean lake: Laguna del Maule, central Chile (36 S).
Carrevedo, M., Frugone, M., Latorre, C., Maldonado, A., Bernardez, P., & Prego, R. et al.
Central Chile is heavily exploited for mineral and water resources, with agriculture and large urban populations all creating intensive landscape use. Few records of past environmental and climate change are available that afford a broader context. To aid in this assessment, we present a 700-year reconstruction from Laguna del Maule (LdM) in the high Andes of central Chile based on sedimentological, geochemical, diatom and pollen analyses. The age model is based on 210Pb/137Cs and 14C dating tied into known volcanic eruptions. Sedimentology consists of organic-rich sediments and diatom oozes with several interspersed volcanic-rich facies and two tephra deposits. Sediment geochemistry exhibits increased productivity (high Br/Ti, biosilica) and more dominant oxic conditions (high Fe/Mn) from AD 1300 to 1400 and from AD 1650 to 1850, likely during periods of relatively lower lake levels and better development of littoral environments. However, during this later period, high elevation vegetation was dominant, indicative of regional cooler/wetter conditions. In contrast, sediments deposited from AD 1850 to 1930 evidence decreased productivity and increased anoxic lake bottom conditions. The ‘Little Ice Age’ (LIA) in LdM is characterized by significant variations in lake dynamics and hydrology with cooler/wetter conditions (AD 1570–1700), major environmental changes in the 18th century and ending at ca. AD 1850. LdM record documents the impact of the LIA in the southern hemisphere and stresses the global nature of this climate period. Large changes in lake dynamics and diatoms assemblages during the 20th century could be related to anthropogenic impacts, but recent changes in climate patterns cannot be excluded.
Año: 2015
Palabras claves: Chile, diatoms, geochemistry, Late Holocene, Little Ice Age, pollen.
Referencia APA: Carrevedo, M., Frugone, M., Latorre, C., Maldonado, A., Bernardez, P., & Prego, R. et al. (2015). A 700-year record of climate and environmental change from a high Andean lake: Laguna del Maule, central Chile (36 S). The Holocene, 25(6), 956-972.
Isolation and Characterization of 12 Microsatellite Loci in Soapbark, Quillaja saponaria (Quillajaceae).
Letelier, L., Harvey, N., Valderrama, A., Stoll, A., & González-Rodríguez, A.
Premise of the study: Microsatellite primers were developed for the endemic Chilean tree Quillaja saponaria (Quillajaceae), a common member of the sclerophyllous Mediterranean forest, to investigate intraspecific patterns of genetic diversity and structure.
Methods and Results: Using an enriched library, 12 polymorphic microsatellite loci were developed in Q. saponaria. All loci consisted of dinucleotide repeats. The average number of alleles per locus was 5.3 (2–13), with a total of 64 alleles recorded in 39 individuals from three populations.
Conclusions: The microsatellite markers described here are the first characterized for Q. saponaria. The polymorphic loci will be useful in studies of genetic diversity and genetic population differentiation in natural populations of this species.
Año: 2015
Palabras claves: Chile, microsatellites, Quillaja saponaria, Quillajaceae, soapbark.
Referencia APA: Letelier, L., Harvey, N., Valderrama, A., Stoll, A., & González-Rodríguez, A. (2015). Isolation and Characterization of 12 Microsatellite Loci in Soapbark, Quillaja saponaria (Quillajaceae). Applications In Plant Sciences, 3(5), 1500024.
Effects of wind-driven spatial structure and environmental heterogeneity on high-altitude wetland macroinvertebrate assemblages with contrasting dispersal modes.
Bertin, A., Alvarez, E., Gouin, N., Gianoli, E., Montecinos, S., & Lek, S. et al.
Dispersal is a major determinant of connectivity between communities that may modulate the importance of environmental and spatial processes on taxonomic composition. While wind is likely to influence transport distance, rate and direction for numerous species, its effects on community composition remain poorly understood.
Using eigenvector-based spatial analyses, we investigated the influence of wind flows, estimated by mesoscale models, on the spatial structure of benthic macroinvertebrate assemblages with contrasting flying abilities in high Andean wetlands (26–32°S). We further quantified the relative importance of local and regional processes through a variation partitioning approach.
The influence of environmental heterogeneity was prevalent and of relatively similar amplitude in both flying and non-flying assemblages. Significant spatial structure more strongly driven by wind flows than by Euclidean schemes was detected for both assemblages. As expected, the highest levels of spatial structure were observed for the flying macroinvertebrate assemblage and they occurred at a much larger spatial scale in this group, suggesting a greater dispersal aptitude of the flying taxa.
Our results show that environmental effects are predominant in shaping the spatial structure of macroinvertebrate communities in high Andean wetlands, as generally found in other systems. They also demonstrate the significance of wind flows in regulating high-altitude wetland macroinvertebrate communities and illustrate the importance of considering adequate spatial models and biological characteristics of species to advance our understanding of community patterns.
Año: 2015
Palabras claves: Benthic macroinvertebrates; dispersal; eigenvector maps; wetlands; wind.
Referencia APA: Bertin, A., Alvarez, E., Gouin, N., Gianoli, E., Montecinos, S., & Lek, S. et al. (2015). Effects of wind-driven spatial structure and environmental heterogeneity on high-altitude wetland macroinvertebrate assemblages with contrasting dispersal modes. Freshwater Biology, 60(2), 297-310.
Updated site compilation of the Latin American Pollen Database
Flantua, S., Hooghiemstra, H., Grimm, E., Behling, H., Bush, M., & González-Arango, C., Gosling, W.D., Ledru, M.P., Lozano-García, S., Maldonado, A., Prieto, A.R., Rull, V., Van Boxel, J.H.
The updated inventory of the Latin American Pollen Database (LAPD) offers a wide range of new insights. This paper presents a systematic compilation of palynological research in Latin America. A comprehensive inventory of publications in peer-reviewed and grey literature shows a major expansion of studies over the last decades. The inventory includes 1379 cores and sections with paleoecological data and more than 4800 modern samples from throughout the continent. Through the years, pollen datasets extend over increasing spans of time and show improved taxonomic and temporal resolution. Currently, these datasets are from 12 modern biomes and 30 countries, covering an altitudinal range of 0 to 6300 m asl. The most densely sampled regions are the Colombian Andes, the southeast coast of Brazil, and Patagonia. Underrepresented biomes are the warm temperate mixed forest (3%), dry forests (3%), and warm temperate rainforest (1%); whereas steppe, tropical rainforest, and cool grass shrublands, such as the páramos, are generally well represented (all > 17%). There are 126 records that span the late Pleistocene to the Last Glacial Maximum transition (21,000 cal yr BP), and > 20% of the records cover the Younger Dryas interval and the Pleistocene/Holocene transition. Reanalysis of numerous sites using multiproxy tools emphasize the informative value of this approach in paleoenvironmental reconstruction. We make suggestions for several pollen sites and regions to be visited again; similarly we identify some key research questions that have yet to be answered. The updated LAPD now provides the platform to support an exciting new phase of global palynological research in which multi-site data are being integrated to address current cutting-edge research questions. The LAPD compilation of sites and the literature database will be available through the Neotoma Paleoecology Database website and a new LAPD website by the end of 2015.
Año: 2015
Palabras claves: Pollen analysis; Latin America; Site distribution patterns; Age control density; Publication history; LAPD.
Referencia APA: Flantua, S., Hooghiemstra, H., Grimm, E., Behling, H., Bush, M., & González-Arango, C., Gosling, W.D., Ledru, M.P., Lozano-García, S., Maldonado, A., Prieto, A.R., Rull, V., Van Boxel, J.H. (2015). Updated site compilation of the Latin American Pollen Database. Review Of Palaeobotany And Palynology, 223, 104-115.
Comparison of DNA extraction methods for polymerase chain reaction amplification of guanaco (Lama guanicoe) fecal DNA samples.
Espinosa, M., Bertin, A., Squeo, F., Cortés, A., & Gouin, N.
Feces-based population genetic studies have become increasingly popular. However, polymerase chain reaction (PCR) amplification rates from fecal material vary depending on the species, populations, loci, and extraction protocols. Here, we assessed the PCR amplification success of three microsatellite markers and a segment of the mitochondrial control region of DNA extracted from field-collected feces of guanaco (Lama guanicoe) using two protocols - Qiagen DNA Stool Kit and 2 cetyltrimethylammonium bromide/phenol:chloroform:isoamyl alcohol (2CTAB/PCI) method. Chelex resin treatment to remove inhibitors was also tested. Our results show that the mitochondrial locus was the most difficult to amplify. PCR success rates
improved for all markers after Chelex treatment of extracted DNA, and 2CTAB/PCI method (95.83%) appeared to perform slightly better than stool kit (91.67%) for the nuclear markers. Amplification success was significantly influenced by the extraction method, Chelex treatment, and locus (P < 0.001) but not by the freshness of the feces (fresh vs old, P = 0.17). The repeatability levels were high without Chelex treatment (> 0.89), but they decreased slightly after treatment for amplification of nuclear markers and markedly after treatment for amplification of the mitochondrial control region. Thus, we showed that Chelex treatment gives high PCR success, especially for nuclear markers, and adequate DNA extraction rates can be achieved from L. guanicoe feces even from non-fresh fecal material. Although not significant, 2CTAB/PCI method tended to provide higher successful amplification rates on a whole set of samples, suggesting that the method could be particularly useful when using small sample sizes.
Año: 2015
Palabras claves: Non-invasive genetics; Mitochondrial control region;Microsatellites; Polymerase chain reaction inhibitors;Chelex treatment; Lama guanicoe.
Referencia APA: Espinosa, M., Bertin, A., Squeo, F., Cortés, A., & Gouin, N. (2015). Comparison of DNA extraction methods for polymerase chain reaction amplification of guanaco (Lama guanicoe) fecal DNA samples. Genetics And Molecular Research, 14(1), 400-406.
Changes of heritability and genetic correlations in production traits over time in red abalone ( Haliotis rufescens ) under culture.
Brokordt, K., Winkler, F., Farías, W., González, R., Castaño, F., Fullsack, P., & Herbinger, C.
Red abalone Haliotis rufescens is one of the most valuable mollusks in the international market, but it has a low growth rate. A breeding program is being developed to increase its growth rate in Chile. We estimated the changes in direct heritability (h2), maternal/common environments heritability (m2) and genetic correlations (rG) of growth traits (shell length and width, total mass, flesh mass and foot protein as an indicator of meat quality) measured during 2 years (every 4 months) from the juvenile stage (27 months) to the adult harvesting age (51 months), in 60 full-sib red abalone families. Heritabilities for growth traits measured in juveniles and young adults (27–35 months of age), were low (0.07–0.17) and not significant. Initial low h2 were associated with significant amounts of maternal/common environmental effects (m2 = 0.4). In young adults and abalone near the harvest age (39–51 months of age) h2 were much higher (0.32–0.75). These results emphasize the importance of multiple estimations of h2 over time. Among meat quality traits, only the h2 for the flesh mass for adults at harvesting age was significant (0.15). We observed strong positive rG (>0.9) between shell sizes (easy to measure) and total and flesh masses (trait more related to market value than shell sizes but harder to measure) for adults at harvesting age. Thus, if the 5% largest 51 month old abalone were selected from the population as broodstock we expect a positively correlated response on flesh mass of 23.4%.
Año: 2015
Palabras claves: Abalone aquaculture; heritability; maternal effects; growth; genetic improvement; Haliotis rufescens.
Referencia APA: Brokordt, K., Winkler, F., Farías, W., González, R., Castaño, F., Fullsack, P., & Herbinger, C. (2015). Changes of heritability and genetic correlations in production traits over time in red abalone (Haliotis rufescens) under culture. Aquaculture Research, 46(9), 2248-2259.
Mitochondrial DNA diversity of feral pigs from Karukinka Natural Park, Tierra del Fuego Island, Chile.
Aravena, P., Skewes, O., & Gouin, N.
: Control or eradication of exotic species is one of the greatest challenges facing biodiversity and ecosystem conservation. Domestic pigs (Sus scrofa domestica) were released and became feral in the southern region of Chilean Tierra del Fuego Island in the 1900s.
Currently, they inhabit part of Karukinka Natural Park, an area of global conservation concern. To gain insight into the control of this invasive species, we analyzed genetic variation in the mitochondrial DNA control region to determine the origin and population subdivision of feral pigs in Tierra del Fuego. Sequences from a sample of 42 feral pigs, 10 domestic pigs from local farms, and references from other countries and commercial breeds revealed 2 highly differentiated populations,
1 in the western and the other in the eastern area of the park, each harboring a different haplotype, suggesting no connectivity between populations. Comparison of these haplotypes with reference sequences from other countries and commercial breeds indicated that feral pigs from Chilean Tierra del Fuego are of European origin, very likely from
2 separate introduction events. The haplotype found in the western feral population was also identified in domestic pigs from a farm. This raises concerns regarding the possible connectivity between stocks from local farms and the wild population. Based on these results, we recommend the development of strategies for controlling the population of this invasive species in Karukinka Natural Park.
Año: 2015
Palabras claves: Feral pigs; Invasive species; mtDNA control region;Sus scrofa; Tierra del Fuego Island.
Referencia APA: Aravena, P., Skewes, O., & Gouin, N. (2015). Mitochondrial DNA diversity of feral pigs from Karukinka Natural Park, Tierra del Fuego Island, Chile. Genetics And Molecular Research, 14(2), 4245-4257.
Tefrocronología en curso superior del valle del río Cisne (44°S), Chile Austral.
Stern, C., De Porras, M., & Maldonado, A.
Based on their petrography and chemistry, 18 tephra analyzed from two lake and bog cores and one outcrop in the upper Río Cisnes valley are believed to have been derived from nine different eruptions of the Mentolat volcano, four of the Melimoyu volcano, and one from the Hudson volcano. Some of these tephra correlate chronologically and petrochemically with previously documented large eruptions of these volcanoes, including the Late-Glacial Ho eruption of Hudson (17,340 cal yrs BP), the mid-Holocene MEN1 eruption of Mentolat (7,710 cal yrs BP), and the Late-Holocene MEL2 eruption of Melimoyu (1,680 cal yrs BP). A Melimoyu-derived tephra from the outcrop occurs in glacial-lacustrine sediments and is considered to pre-date the Last Glacial Maximum (>19,670 cal yrs BP). The data suggest that none of the tephra were produced by explosive eruptions of the Maca, Cay and Yanteles volcanoes.
Año: 2015
Palabras claves: Tephra, Tephrochronology, Tephrostratigraphy, Volcanism, Andes, Chile.
Referencia APA: Stern, C., De Porras, M., & Maldonado, A. (2015). Tefrocronología en curso superior del valle del río Cisne (44°S), Chile Austral. Andean Geology, 42(2).